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pericytes hpl pcs  (PromoCell)


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    PromoCell pericytes hpl pcs
    CD140B+CD44+ cells are derived from human embryonic stem cells (hESCs) in a matrix-dependent manner. (A): Schematic representation of matrix-dependent differentiation process for CD140B+CD44+ cells. (B): Two-color flow cytometry was used to check mesodermal specification following the differentiation schedule. All single cells were labeled with antibodies against CD140B and CD44. Obtained cells are distinguished from CD140B− and CD140B+ by the horizontal lines in each plot. Cases #1 and #2 are typical representative aspects, depending on a different starting H9-hESC line. After collagen-dependent natural selection through a simple medium change, the attached CD140B+ population showed a strong CD44 expression at Day 7 (A). Values in each quadrant plot represent percentage of population, mean ±SD (n = 6). (C, D): The change of gene context by collagen-dependent attachment step showed a big difference. Differential gene expression profiling was performed among H9-hESCs, embryoid bodies (EBs) induced by bone morphogenetic protein 4 (BMP4) (EBs) and naturally selected CD140B+CD44+ population at Day 7 (perivascular progenitor cells, PVPCs, CD140B+CD44+ population) from three independent batches. (C): Heatmap of each group. Each column represents a single microarray analysis. (D): Hierarchical clustering analysis of the global gene expression proϕiles using the average linkage and the Pearson distance. (E): Expanded CD140B+CD44+ population exhibited a unique cell morphology. Scale bar = 20 μm. (F): The cell proliferation was monitored with the CCK-8 assay at different time points. Data are means of three separated experiments ± SD. Abbreviations: BM-MSC, human bone marrow-derived mesenchyme stem cells; h, hour; <t>hPL-PC,</t> human placenta-derived <t>pericyte;</t> MEF, mouse embryonic fibroblast; OD, optical density.
    Pericytes Hpl Pcs, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pericytes hpl pcs/product/PromoCell
    Average 95 stars, based on 1 article reviews
    pericytes hpl pcs - by Bioz Stars, 2026-05
    95/100 stars

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    1) Product Images from "Perivascular Progenitor Cells Derived From Human Embryonic Stem Cells Exhibit Functional Characteristics of Pericytes and Improve the Retinal Vasculature in a Rodent Model of Diabetic Retinopathy"

    Article Title: Perivascular Progenitor Cells Derived From Human Embryonic Stem Cells Exhibit Functional Characteristics of Pericytes and Improve the Retinal Vasculature in a Rodent Model of Diabetic Retinopathy

    Journal: Stem Cells Translational Medicine

    doi: 10.5966/sctm.2015-0342

    CD140B+CD44+ cells are derived from human embryonic stem cells (hESCs) in a matrix-dependent manner. (A): Schematic representation of matrix-dependent differentiation process for CD140B+CD44+ cells. (B): Two-color flow cytometry was used to check mesodermal specification following the differentiation schedule. All single cells were labeled with antibodies against CD140B and CD44. Obtained cells are distinguished from CD140B− and CD140B+ by the horizontal lines in each plot. Cases #1 and #2 are typical representative aspects, depending on a different starting H9-hESC line. After collagen-dependent natural selection through a simple medium change, the attached CD140B+ population showed a strong CD44 expression at Day 7 (A). Values in each quadrant plot represent percentage of population, mean ±SD (n = 6). (C, D): The change of gene context by collagen-dependent attachment step showed a big difference. Differential gene expression profiling was performed among H9-hESCs, embryoid bodies (EBs) induced by bone morphogenetic protein 4 (BMP4) (EBs) and naturally selected CD140B+CD44+ population at Day 7 (perivascular progenitor cells, PVPCs, CD140B+CD44+ population) from three independent batches. (C): Heatmap of each group. Each column represents a single microarray analysis. (D): Hierarchical clustering analysis of the global gene expression proϕiles using the average linkage and the Pearson distance. (E): Expanded CD140B+CD44+ population exhibited a unique cell morphology. Scale bar = 20 μm. (F): The cell proliferation was monitored with the CCK-8 assay at different time points. Data are means of three separated experiments ± SD. Abbreviations: BM-MSC, human bone marrow-derived mesenchyme stem cells; h, hour; hPL-PC, human placenta-derived pericyte; MEF, mouse embryonic fibroblast; OD, optical density.
    Figure Legend Snippet: CD140B+CD44+ cells are derived from human embryonic stem cells (hESCs) in a matrix-dependent manner. (A): Schematic representation of matrix-dependent differentiation process for CD140B+CD44+ cells. (B): Two-color flow cytometry was used to check mesodermal specification following the differentiation schedule. All single cells were labeled with antibodies against CD140B and CD44. Obtained cells are distinguished from CD140B− and CD140B+ by the horizontal lines in each plot. Cases #1 and #2 are typical representative aspects, depending on a different starting H9-hESC line. After collagen-dependent natural selection through a simple medium change, the attached CD140B+ population showed a strong CD44 expression at Day 7 (A). Values in each quadrant plot represent percentage of population, mean ±SD (n = 6). (C, D): The change of gene context by collagen-dependent attachment step showed a big difference. Differential gene expression profiling was performed among H9-hESCs, embryoid bodies (EBs) induced by bone morphogenetic protein 4 (BMP4) (EBs) and naturally selected CD140B+CD44+ population at Day 7 (perivascular progenitor cells, PVPCs, CD140B+CD44+ population) from three independent batches. (C): Heatmap of each group. Each column represents a single microarray analysis. (D): Hierarchical clustering analysis of the global gene expression proϕiles using the average linkage and the Pearson distance. (E): Expanded CD140B+CD44+ population exhibited a unique cell morphology. Scale bar = 20 μm. (F): The cell proliferation was monitored with the CCK-8 assay at different time points. Data are means of three separated experiments ± SD. Abbreviations: BM-MSC, human bone marrow-derived mesenchyme stem cells; h, hour; hPL-PC, human placenta-derived pericyte; MEF, mouse embryonic fibroblast; OD, optical density.

    Techniques Used: Derivative Assay, Flow Cytometry, Labeling, Selection, Expressing, Microarray, CCK-8 Assay

    Naturally selected CD140B+CD44+ cells show the characteristics of multipotent perivascular progenitor cells (PVPCs). (A): Expanded CD140B+CD44+ cells (human embryonic stem cells derived from PVPCs; hESC-PVPCs) were labeled with the indicated antibodies. The overlaid open histogram displays immunoglobulin control against each target. Values in each quadrant plot represent percentage of population, mean ± SD (n = 8). (B): Generalized pericyte marker, NG2 (green) expressed in both hESC-PVPCs and human primary pericytes (hPL-PC). Nuclear DNA was stained with 4′,6-diamidino-2-phenylindole (blue). (C): Skeletogenic differentiation potential of hESC-PVPCs was shown in the differentiation condition of adipogenesis (left, Oil red staining) and osteogenesis (right, Alizarin red staining). (D): Smooth muscle cell (SMC) differentiation potential of hESC-PVPCs was shown in SMC differentiation media (SMDM). Quantitative polymerase chain reaction values represent mean (n = 3) ± SD (∗∗∗, p < .0001). SMC-specific markers, α-SMA (green), and CNN (red) were detected in hESC-PVPC after 6 days in SMDM differentiation. (E): Dye transfer (circle in the plot, yellow-green) increased in a coculture of DiI-labeled hESC-PVPC (PVPC-DiI, red) and Calcein-labeled other vascular lineages (upper, human umbilical vein endothelial cell, HUVEC-Calcein; lower, umbilical artery smooth muscle cell, UASMC-Calcein, green). Values in each plot represent percentage of population, mean ± SD (n = 3). Dye-transferred hESC-PVPCs were also detected on culture well (white dots). (F, G): Perivascular localization of hESC-PVPC (PVPC, DiI, red) was confirmed in three-dimensional fibrin gel bead assay with HUVEC (DiO, green). Magnified view (G) of rectangular region (F), and orthogonal projection images (white dotted lines 1 and 2). All scale bars = 100 μm. Abbreviations: α-SMA, α-smooth muscle actin; CNN, Calponin 1; d, days.
    Figure Legend Snippet: Naturally selected CD140B+CD44+ cells show the characteristics of multipotent perivascular progenitor cells (PVPCs). (A): Expanded CD140B+CD44+ cells (human embryonic stem cells derived from PVPCs; hESC-PVPCs) were labeled with the indicated antibodies. The overlaid open histogram displays immunoglobulin control against each target. Values in each quadrant plot represent percentage of population, mean ± SD (n = 8). (B): Generalized pericyte marker, NG2 (green) expressed in both hESC-PVPCs and human primary pericytes (hPL-PC). Nuclear DNA was stained with 4′,6-diamidino-2-phenylindole (blue). (C): Skeletogenic differentiation potential of hESC-PVPCs was shown in the differentiation condition of adipogenesis (left, Oil red staining) and osteogenesis (right, Alizarin red staining). (D): Smooth muscle cell (SMC) differentiation potential of hESC-PVPCs was shown in SMC differentiation media (SMDM). Quantitative polymerase chain reaction values represent mean (n = 3) ± SD (∗∗∗, p < .0001). SMC-specific markers, α-SMA (green), and CNN (red) were detected in hESC-PVPC after 6 days in SMDM differentiation. (E): Dye transfer (circle in the plot, yellow-green) increased in a coculture of DiI-labeled hESC-PVPC (PVPC-DiI, red) and Calcein-labeled other vascular lineages (upper, human umbilical vein endothelial cell, HUVEC-Calcein; lower, umbilical artery smooth muscle cell, UASMC-Calcein, green). Values in each plot represent percentage of population, mean ± SD (n = 3). Dye-transferred hESC-PVPCs were also detected on culture well (white dots). (F, G): Perivascular localization of hESC-PVPC (PVPC, DiI, red) was confirmed in three-dimensional fibrin gel bead assay with HUVEC (DiO, green). Magnified view (G) of rectangular region (F), and orthogonal projection images (white dotted lines 1 and 2). All scale bars = 100 μm. Abbreviations: α-SMA, α-smooth muscle actin; CNN, Calponin 1; d, days.

    Techniques Used: Derivative Assay, Labeling, Marker, Staining, Real-time Polymerase Chain Reaction



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    pericytes hpl pcs  (PromoCell)
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    PromoCell pericytes hpl pcs
    CD140B+CD44+ cells are derived from human embryonic stem cells (hESCs) in a matrix-dependent manner. (A): Schematic representation of matrix-dependent differentiation process for CD140B+CD44+ cells. (B): Two-color flow cytometry was used to check mesodermal specification following the differentiation schedule. All single cells were labeled with antibodies against CD140B and CD44. Obtained cells are distinguished from CD140B− and CD140B+ by the horizontal lines in each plot. Cases #1 and #2 are typical representative aspects, depending on a different starting H9-hESC line. After collagen-dependent natural selection through a simple medium change, the attached CD140B+ population showed a strong CD44 expression at Day 7 (A). Values in each quadrant plot represent percentage of population, mean ±SD (n = 6). (C, D): The change of gene context by collagen-dependent attachment step showed a big difference. Differential gene expression profiling was performed among H9-hESCs, embryoid bodies (EBs) induced by bone morphogenetic protein 4 (BMP4) (EBs) and naturally selected CD140B+CD44+ population at Day 7 (perivascular progenitor cells, PVPCs, CD140B+CD44+ population) from three independent batches. (C): Heatmap of each group. Each column represents a single microarray analysis. (D): Hierarchical clustering analysis of the global gene expression proϕiles using the average linkage and the Pearson distance. (E): Expanded CD140B+CD44+ population exhibited a unique cell morphology. Scale bar = 20 μm. (F): The cell proliferation was monitored with the CCK-8 assay at different time points. Data are means of three separated experiments ± SD. Abbreviations: BM-MSC, human bone marrow-derived mesenchyme stem cells; h, hour; <t>hPL-PC,</t> human placenta-derived <t>pericyte;</t> MEF, mouse embryonic fibroblast; OD, optical density.
    Pericytes Hpl Pcs, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pericytes hpl pcs/product/PromoCell
    Average 95 stars, based on 1 article reviews
    pericytes hpl pcs - by Bioz Stars, 2026-05
    95/100 stars
    		  Buy from Supplier

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    CD140B+CD44+ cells are derived from human embryonic stem cells (hESCs) in a matrix-dependent manner. (A): Schematic representation of matrix-dependent differentiation process for CD140B+CD44+ cells. (B): Two-color flow cytometry was used to check mesodermal specification following the differentiation schedule. All single cells were labeled with antibodies against CD140B and CD44. Obtained cells are distinguished from CD140B− and CD140B+ by the horizontal lines in each plot. Cases #1 and #2 are typical representative aspects, depending on a different starting H9-hESC line. After collagen-dependent natural selection through a simple medium change, the attached CD140B+ population showed a strong CD44 expression at Day 7 (A). Values in each quadrant plot represent percentage of population, mean ±SD (n = 6). (C, D): The change of gene context by collagen-dependent attachment step showed a big difference. Differential gene expression profiling was performed among H9-hESCs, embryoid bodies (EBs) induced by bone morphogenetic protein 4 (BMP4) (EBs) and naturally selected CD140B+CD44+ population at Day 7 (perivascular progenitor cells, PVPCs, CD140B+CD44+ population) from three independent batches. (C): Heatmap of each group. Each column represents a single microarray analysis. (D): Hierarchical clustering analysis of the global gene expression proϕiles using the average linkage and the Pearson distance. (E): Expanded CD140B+CD44+ population exhibited a unique cell morphology. Scale bar = 20 μm. (F): The cell proliferation was monitored with the CCK-8 assay at different time points. Data are means of three separated experiments ± SD. Abbreviations: BM-MSC, human bone marrow-derived mesenchyme stem cells; h, hour; hPL-PC, human placenta-derived pericyte; MEF, mouse embryonic fibroblast; OD, optical density.

    Journal: Stem Cells Translational Medicine

    Article Title: Perivascular Progenitor Cells Derived From Human Embryonic Stem Cells Exhibit Functional Characteristics of Pericytes and Improve the Retinal Vasculature in a Rodent Model of Diabetic Retinopathy

    doi: 10.5966/sctm.2015-0342

    Figure Lengend Snippet: CD140B+CD44+ cells are derived from human embryonic stem cells (hESCs) in a matrix-dependent manner. (A): Schematic representation of matrix-dependent differentiation process for CD140B+CD44+ cells. (B): Two-color flow cytometry was used to check mesodermal specification following the differentiation schedule. All single cells were labeled with antibodies against CD140B and CD44. Obtained cells are distinguished from CD140B− and CD140B+ by the horizontal lines in each plot. Cases #1 and #2 are typical representative aspects, depending on a different starting H9-hESC line. After collagen-dependent natural selection through a simple medium change, the attached CD140B+ population showed a strong CD44 expression at Day 7 (A). Values in each quadrant plot represent percentage of population, mean ±SD (n = 6). (C, D): The change of gene context by collagen-dependent attachment step showed a big difference. Differential gene expression profiling was performed among H9-hESCs, embryoid bodies (EBs) induced by bone morphogenetic protein 4 (BMP4) (EBs) and naturally selected CD140B+CD44+ population at Day 7 (perivascular progenitor cells, PVPCs, CD140B+CD44+ population) from three independent batches. (C): Heatmap of each group. Each column represents a single microarray analysis. (D): Hierarchical clustering analysis of the global gene expression proϕiles using the average linkage and the Pearson distance. (E): Expanded CD140B+CD44+ population exhibited a unique cell morphology. Scale bar = 20 μm. (F): The cell proliferation was monitored with the CCK-8 assay at different time points. Data are means of three separated experiments ± SD. Abbreviations: BM-MSC, human bone marrow-derived mesenchyme stem cells; h, hour; hPL-PC, human placenta-derived pericyte; MEF, mouse embryonic fibroblast; OD, optical density.

    Article Snippet: Both human placenta-derived pericytes (hPL-PCs) and human bone marrow-derived MSCs (BM-MSCs) were obtained from PromoCell (Heidelberg, Germany, http://www.promocell.com/ ).

    Techniques: Derivative Assay, Flow Cytometry, Labeling, Selection, Expressing, Microarray, CCK-8 Assay

    Naturally selected CD140B+CD44+ cells show the characteristics of multipotent perivascular progenitor cells (PVPCs). (A): Expanded CD140B+CD44+ cells (human embryonic stem cells derived from PVPCs; hESC-PVPCs) were labeled with the indicated antibodies. The overlaid open histogram displays immunoglobulin control against each target. Values in each quadrant plot represent percentage of population, mean ± SD (n = 8). (B): Generalized pericyte marker, NG2 (green) expressed in both hESC-PVPCs and human primary pericytes (hPL-PC). Nuclear DNA was stained with 4′,6-diamidino-2-phenylindole (blue). (C): Skeletogenic differentiation potential of hESC-PVPCs was shown in the differentiation condition of adipogenesis (left, Oil red staining) and osteogenesis (right, Alizarin red staining). (D): Smooth muscle cell (SMC) differentiation potential of hESC-PVPCs was shown in SMC differentiation media (SMDM). Quantitative polymerase chain reaction values represent mean (n = 3) ± SD (∗∗∗, p < .0001). SMC-specific markers, α-SMA (green), and CNN (red) were detected in hESC-PVPC after 6 days in SMDM differentiation. (E): Dye transfer (circle in the plot, yellow-green) increased in a coculture of DiI-labeled hESC-PVPC (PVPC-DiI, red) and Calcein-labeled other vascular lineages (upper, human umbilical vein endothelial cell, HUVEC-Calcein; lower, umbilical artery smooth muscle cell, UASMC-Calcein, green). Values in each plot represent percentage of population, mean ± SD (n = 3). Dye-transferred hESC-PVPCs were also detected on culture well (white dots). (F, G): Perivascular localization of hESC-PVPC (PVPC, DiI, red) was confirmed in three-dimensional fibrin gel bead assay with HUVEC (DiO, green). Magnified view (G) of rectangular region (F), and orthogonal projection images (white dotted lines 1 and 2). All scale bars = 100 μm. Abbreviations: α-SMA, α-smooth muscle actin; CNN, Calponin 1; d, days.

    Journal: Stem Cells Translational Medicine

    Article Title: Perivascular Progenitor Cells Derived From Human Embryonic Stem Cells Exhibit Functional Characteristics of Pericytes and Improve the Retinal Vasculature in a Rodent Model of Diabetic Retinopathy

    doi: 10.5966/sctm.2015-0342

    Figure Lengend Snippet: Naturally selected CD140B+CD44+ cells show the characteristics of multipotent perivascular progenitor cells (PVPCs). (A): Expanded CD140B+CD44+ cells (human embryonic stem cells derived from PVPCs; hESC-PVPCs) were labeled with the indicated antibodies. The overlaid open histogram displays immunoglobulin control against each target. Values in each quadrant plot represent percentage of population, mean ± SD (n = 8). (B): Generalized pericyte marker, NG2 (green) expressed in both hESC-PVPCs and human primary pericytes (hPL-PC). Nuclear DNA was stained with 4′,6-diamidino-2-phenylindole (blue). (C): Skeletogenic differentiation potential of hESC-PVPCs was shown in the differentiation condition of adipogenesis (left, Oil red staining) and osteogenesis (right, Alizarin red staining). (D): Smooth muscle cell (SMC) differentiation potential of hESC-PVPCs was shown in SMC differentiation media (SMDM). Quantitative polymerase chain reaction values represent mean (n = 3) ± SD (∗∗∗, p < .0001). SMC-specific markers, α-SMA (green), and CNN (red) were detected in hESC-PVPC after 6 days in SMDM differentiation. (E): Dye transfer (circle in the plot, yellow-green) increased in a coculture of DiI-labeled hESC-PVPC (PVPC-DiI, red) and Calcein-labeled other vascular lineages (upper, human umbilical vein endothelial cell, HUVEC-Calcein; lower, umbilical artery smooth muscle cell, UASMC-Calcein, green). Values in each plot represent percentage of population, mean ± SD (n = 3). Dye-transferred hESC-PVPCs were also detected on culture well (white dots). (F, G): Perivascular localization of hESC-PVPC (PVPC, DiI, red) was confirmed in three-dimensional fibrin gel bead assay with HUVEC (DiO, green). Magnified view (G) of rectangular region (F), and orthogonal projection images (white dotted lines 1 and 2). All scale bars = 100 μm. Abbreviations: α-SMA, α-smooth muscle actin; CNN, Calponin 1; d, days.

    Article Snippet: Both human placenta-derived pericytes (hPL-PCs) and human bone marrow-derived MSCs (BM-MSCs) were obtained from PromoCell (Heidelberg, Germany, http://www.promocell.com/ ).

    Techniques: Derivative Assay, Labeling, Marker, Staining, Real-time Polymerase Chain Reaction